Simultaneous Measurement of Biophysical Properties and Position of Single Cells in a Microdevice

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Dahou Yang, Ye Ai

Tracking the lateral position of single cells and particles plays an important role in evaluating the efficiency of microfluidic cell focusing, separation and sorting. Traditionally, the performance of microfluidic cell separation and sorting is evaluated either by analyzing the input and collected output samples requiring extra multiple steps of off-chip analysis or the use of expensive equipment (e.g., flow cytometry), or by detecting the lateral positions of cells using an expensive high-speed imaging setup with intricate image processing algorithms or laborious manual analysis. Hence, there is a great need to develop a simple approach for the lateral position measurement of flowing particles.

Schematic design of the electrical sensing region in the microfluidic impedance cytometry device. The lateral position of single particles or cells flowing through the N-shaped electrodes can be calculated from the electrical signal and the geometry relationship among the positions of the flowing particles, electrodes and microchannel.

You can find more details at https://www.sutd.edu.sg/Research/Research-News/2019/11/Simultaneous-measurement-of-biophysical-properties

2021-09-28T11:24:47+00:00