SUTD Researchers Discover Fluorescence-activated Sorting at Single-droplet Level using Focused Sound Waves

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SUTD: Peixian Li, Zhichao Ma, Yinning Zhou, Ye Ai
University of Melbourne: David J. Collins
SIMTech: Zhenfeng Wang

SUTD researchers developed a new microfluidic fluorescence-activated droplet sorting system that can isolate single-cell droplets with high accuracy and high yield using a detachable surface acoustic wave transducer. This novel microfluidic system can be readily integrated with droplet-based single-cell sequencing platform to achieve highly efficient single-cell genetic analysis.

In recent years, rapid progress in the development of single-cell sequencing has provided numerous important insights for understanding the functionality and variation within cell types. Droplet-based microfluidic system has emerged as a very powerful and effective tool to isolate and analyze biological cells at the single-cell level. However, existing droplet generation is a random process that captures cells at a single-cell encapsulation rate of generally lower than 5%. Hence, there is a great need to develop a platform which can isolate single-cell droplets at high yield.

An acoustic fluorescence-activated droplet sorting (FADS) system that can achieve accurate isolation of single-cell droplets for high-yield single cell sequencing.

You can find more details at https://www.sutd.edu.sg/Research/Research-News/2019/12/SUTD-fluorescence-activated-sorting-single-droplet

2021-09-28T11:18:56+00:00